If it's the only thing on the cable, it should either be jumpered as a master or left unjumpered. The docs should tell, but in no case should it be a slave.
On the off-change that you might have a cable-select cable, have you tried it in both connectors?
Next option, as I said, would be to swap something else in to make sure the cable is working fine.
You might also unplug the cable (from the mobo and the drive) and plug it back in. I've been nailed by loose connections before.
You might also need to check the BIOS and make sure the second IDE channel hasn't been disabled or something.
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