The encounter of the 6 people that turn themselves into your party is most likely NOT connected to the Twisted Rune, Slavers, etc. If you examine their loot after you kill them, you will find that they were most probably Rakshasa, who are known to work with Irenicus on occasion. I have never heard of any ties between Irenicus and the Twisted Rune & Co.
(Although why Irenicus would bother to send Rakshasa assassins after you is another matter, since he knows that you'll die on your own soon enough and doesn't give a flying fart about you anyway.)
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Volothamp's Comeuppance
Everything you ever needed to know about the entire Baldur's Gate series......except spoilers.
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